<p>a–c: insulin secretion after 1, 10, and 20 day cultures, respectively. (mean±SEM: n = 3, *:p<0.05). d: microscopic morphology, scale bar: 20 µm A–E: after 1 day culture. F–J: after 10 days of culture. K–O: after 20 days of culture.</p
<p>MIN6 cells transiently transfected with insulin-HT plus calnexin-YFP, insulin-HT plus M6PR-EGFP, ...
<p>(A) Cellular insulin content was examined after MIN6 cells were incubated in normoxia (20% O<sub>...
<p>Cells were grown for 24 h with the addition of either vehicle (water) or 20 nM insulin. To facili...
<p>Insulin released into culture medium from day 0 to 3 and from day 12 to 15 and also in extracts c...
<p>A: Morphological changes of the islets cultured with or without MSC at 4 d after starting the cul...
<p>A, MIN6/insulin-HT cells were incubated with 100 nM HT-R110 probe for 16 hours. The cells were th...
<p>(A) Measurement of glucose-stimulated insulin secretion from NPLCs transduced with Ad-GFP or Ad-P...
<p>A: Schematic representation of the culture procedure used to derive the RYAS41 cell line. P repre...
<p>(A) αTC1 cells were co-cultured with MIN6 β cells (n = 16), αTC1 (n = 16), CHO-NEO (n = 12) and m...
<p>Cells were exposed to vehicle or 1–100 nM insulin for 48 h or to 10 nM insulin for 6–48 h. (A) Th...
<p>Glucose-stimulated insulin secretion was tested at three subsequent concentrations of glucose (3 ...
<p>(A) Staining of viable (green) <i>versus</i> dead (red) cells in cultures of naked islets (CTR), ...
The electrofusion-derived rat insulin-secreting cell line BRIN-BD11 was cultured in five different c...
<p>A and B, MIN6/insulin-HT cells were labeled with HT-TMR (A) or HT-biotin probe (B) for the indica...
<p>Significant insulin concentration was observed in MPE–treated IPCs. To normalize the amount of in...
<p>MIN6 cells transiently transfected with insulin-HT plus calnexin-YFP, insulin-HT plus M6PR-EGFP, ...
<p>(A) Cellular insulin content was examined after MIN6 cells were incubated in normoxia (20% O<sub>...
<p>Cells were grown for 24 h with the addition of either vehicle (water) or 20 nM insulin. To facili...
<p>Insulin released into culture medium from day 0 to 3 and from day 12 to 15 and also in extracts c...
<p>A: Morphological changes of the islets cultured with or without MSC at 4 d after starting the cul...
<p>A, MIN6/insulin-HT cells were incubated with 100 nM HT-R110 probe for 16 hours. The cells were th...
<p>(A) Measurement of glucose-stimulated insulin secretion from NPLCs transduced with Ad-GFP or Ad-P...
<p>A: Schematic representation of the culture procedure used to derive the RYAS41 cell line. P repre...
<p>(A) αTC1 cells were co-cultured with MIN6 β cells (n = 16), αTC1 (n = 16), CHO-NEO (n = 12) and m...
<p>Cells were exposed to vehicle or 1–100 nM insulin for 48 h or to 10 nM insulin for 6–48 h. (A) Th...
<p>Glucose-stimulated insulin secretion was tested at three subsequent concentrations of glucose (3 ...
<p>(A) Staining of viable (green) <i>versus</i> dead (red) cells in cultures of naked islets (CTR), ...
The electrofusion-derived rat insulin-secreting cell line BRIN-BD11 was cultured in five different c...
<p>A and B, MIN6/insulin-HT cells were labeled with HT-TMR (A) or HT-biotin probe (B) for the indica...
<p>Significant insulin concentration was observed in MPE–treated IPCs. To normalize the amount of in...
<p>MIN6 cells transiently transfected with insulin-HT plus calnexin-YFP, insulin-HT plus M6PR-EGFP, ...
<p>(A) Cellular insulin content was examined after MIN6 cells were incubated in normoxia (20% O<sub>...
<p>Cells were grown for 24 h with the addition of either vehicle (water) or 20 nM insulin. To facili...