Determination of secreted (A) and secreted versus intracellular (B) insulin in P19 undifferentiated EC cells (P19), spontaneous differentiated EBs (EB) and the MPE-treated cells.

<p>Significant insulin concentration was observed in MPE–treated IPCs. To normalize the amount of insulin secretion, the total protein of the cells in each well was measured by the Bradford method. The experiment was performed in triplicate. Each value represents mean ± SD.