Characterization of SR1423 by endocrine pancreatic markers expression and quantification.

SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 differentiation labeled for Pdx1 (red) and Nkx6.1 (green) expression. (B) SR1423 differentiation labeled by Pdx1 (red) and NeuroD1 (green). (C) SR1423 differentiation labeled for Insulin (green) and C-peptide (red). (D) SR1423 differentiation labeled for Pdx1 (red) and Glucagon (green). “Merge” images include nuclear stain (blue). All images were taken after 28 days of differentiation under 40X original magnification (n = 10). Scale bar 100 μm. (E) Flow cytometry of SR1423 differentiated for 24 days. Cells stained with anti-Insulin (green), anti-Pdx1 (blue), anti-Nkx6.1 (red), and anti-Glucagon (orange) displayed with overlays of negative controls (black) in each panel. Quadrants are labeled with percentages of experimental cells within the spider-gated region (n = 5000 cells). An example of three repeated experiments is shown.