CFA treatment enhances the percentage of CD4⁺CD25⁺ regulatory T cells expressing CD103 and granzyme B.

<p>Spleen cells were harvested from 11 week-old B6 or NOD mice injected with PBS or CFA three weeks earlier. Cells were labeled with anti-CD4, CD25 and CD103 antibodies and analyzed by FACS® gating on CD4⁺CD25⁺ cells. Each point represents an individual animal. An * indicates a significant difference from PBS-treated NOD at <i>p</i><0.05 (A). CD4⁺CD25⁺ T cells from 11 week-old NOD mice were pooled and cultured for 40 hrs with irradiated T cell-depleted spleen cells (APC) pooled (4–5 mice) from 11 week-old B6 or PBS- (NOD) or CFA-treated NOD (CFA-NOD) and anti-CD3 antibody. Cells were labeled with anti-CD4 and CD25 antibodies, and intracellularly labeled with anti-granzyme B antibody then analyzed by FACS® by gating on CD4⁺CD25⁺ cells. Representative results from one of three experiments are shown (B).