CFA treatment enhances Foxp3 expression by CD4⁺CD25⁺ regulatory T cells <i>in vivo</i>.

<p>CD4⁺CD25⁺ regulatory T cells were cultured for 24 hrs with APC (irradiated T cell-depleted spleen cells) pooled from 11 week-old B6 or NOD mice injected with PBS or CFA three weeks earlier and anti-CD3 antibody (A). Spleen (B), pancreatic LN (pLN) (C) and pancreas (D) were harvested from 11 week-old B6 or NOD mice injected with PBS or CFA three weeks earlier. Cells were labeled with anti-CD4, CD25 and Foxp3 antibodies and analyzed by FACS® gating on CD4⁺CD25⁺ cells (A–D). Each point represents an individual animal (B–D). An * indicates a significant difference from PBS-treated NOD (<i>p</i><0.05).