APC from CFA-treated NOD mice effectively induce regulatory cell function <i>in vitro</i>.

<p>CD4⁺CD25⁻ responder cells from B6 mice were cultured with irradiated spleen cells (APC) from individual 30 week-old B6, Non-Sick (NS) CFA-treated or Sick NOD mice, anti-CD3 and either with or without CD4⁺CD25⁺ cells from B6 mice (1∶1 regulatory∶responder ratio; A & B). CD4⁺CD25⁻ responder cells from NOD mice were cultured with irradiated spleen cells (APC) from individual 11 week-old NOD mice injected with PBS or CFA three weeks earlier or B6 mice, anti-CD3 and either with or without CD4⁺CD25⁺ cells from NOD mice (1∶1 regulatory∶responder ratio; C & D). Raw cpm data are shown for one representative animal (A & C), and percent inhibition is shown where each point represents an individual animal (B & D). * and ** indicate significant differences from the respective proliferation controls (i.e., responders alone; A & C), or B6 APC (B) at <i>p</i><0.001 and p<0.05, respectively.