Effect of LMP1 expression on the interaction of E-cadherin and β-catenin.

<p>Two different methods were used to assess the effect of LMP1 on this interaction. Firstly, E-cadherin was immunoprecipated from the cells stably expressing either LMP1 or EGFP. These immunoprecipitates were then resolved on SDS-PAGE gel followed by immunoblotting (Panel A). These immunoblots were probed with antibodies specific for E-cadherin, β-catenin and α-catenin. In the second strategy, live HaCaT cells stably expressing either LMP1 or EGFP were initially surface labelled with biotin and then immunoprecipitated with anti- E-cadherin or anti-β-catenin antibodies. These immunoprecipitates were then resolved on SDS-PAGE gel followed by immunoblotting (Panel A). These immunoblots were probed with either streptavidin or antibodies specific for E-cadherin and β-catenin.