E-cadherin recruits SCRIB to form an E-cadherin/SCRIB/LGN complex to connect cell polarity and spindle positioning.

(A) Co-IP experiments using RWPE-1 cell lysates suggest that endogenous E-cadherin interacts with SCRIB and LGN. (B) Endogenous SCRIB co-immunoprecipitates with the E-cadherin/LGN protein complex. (C) Co-IP assays indicate that endogenous SCRIB, E-cadherin and LGN form a ternary complex. (D) The interaction between SCRIB and LGN is suppressed by E-cadherin knockdown. (E) SCRIB knockdown attenuates the association between LGN and E-cadherin. (F) Diagram of SCRIB fragments used to identify E-cadherin and LGN binding domains. (G) Pull down assays indicate the fragment containing PDZ domain of SCRIB binds to E-cadherin and LGN.