Expression of cadherins and CAMs (cell adhesion molecules).

(A) Comparison of the relative amounts of Cadherins expressed in the SLGC lines indicated (data from proteome arrays). Based on the assumption that the antibodies spotted on the filters of the proteome arrays possessed similar KDs, the relative expression of the cadherins was calculated. The sum of the signals of all cadherins was arbitrary set at 100%. The bars indicate to what percentage the individual cadherins contribute to this expression. C4, C11, and C13, cadherins 4, 11 and 13; E-C, E-cadherin; N-C, N-cadherin; P-C, placental cadherin; VE-C, VE-(vascular endothelial) cadherin/CD144. (B) Quantification of Western blot analyzes investigating the expression of N-cadherin in the SLGC lines indicated. Bars represent the mean expression normalized against Actin, whiskers represent the variation between technical replicates. (C) Similar experiment as in Fig 5 using biological replicates of the various SLGC lines (flow cytometry analysis). The established cell lines U87 (glioblastoma) and/or CaCo2 (colon carcinoma), which lack stemness were used as references. The antibodies bind to the VE-cadherin/CD144 and PECAM-1 (platelet endothelial cell adhesion molecule -1)/CD31, respectively. Bars depict the percentage of positive cells determined in one representative experiment. (D) Comparison of the relative amounts of CAMs expressed in the SLGC lines indicated (data from proteome arrays). The sum of the signals of all CAMs was arbitrary set at 100%. The bars indicate to what percentage the individual CAMs contribute to the total CAM expression. Cea-CAM-5 or -1, Carcinoembryonic antigen-related cell adhesion molecule -5 or -1; CHL-1, cell adhesion molecule L1-like; N-CAM, nerve cell adhesion molecule; VCAM, Vascular cell adhesion protein 1; PECAM, platelet endothelial cell adhesion molecule; ICAM-2, intercellular adhesion molecule 2; Neurotrimin, GPI-anchored cell adhesion molecule that might promote neurite outgrowth; NCAM-1, neural adhesion molecule-1; NCAM-L1, neural cell adhesion molecule L1; EpCAM, epithelial cell adhesion molecule. (TIF)