Inflammation influences the membrane-localized β-catenin and E-cadherin interaction.

<p><b>A.</b> β-catenin and <b>B.</b> p-β-catenin^(S552) localizations at plasma membrane (arrows) are decreased with CM treatment (500 pg/ml TNFα for 3 h) (magnification 60X), and the β-catenin and p-β-catenin^(S552) localize into cell cytoplasm. <b>C.</b> The loss of membrane-localized β-catenin and E-cadherin interaction at the cell membrane was evidenced when immunoprecipitation (IP) time course was performed. <b>D.</b> A substantial increase in cytoplasmic and nuclear translocated β-catenin after CM treatments were also confirmed in the sub-cellular fractionated cell lysates. E-cadherin, H2A and GAPDH levels were also confirmed not changing after 6 h of treatment as controls for fractions. Memb: Membrane fraction, Nuc: Nuclear fraction, Cyto: Cytoplasmic fraction. <b>E.</b> Furthermore, total ubiquitination as well as β-catenin expression levels are increased in CM treated LNCaP cells whereas β-catenin ubiquitination is decreased. The antibodies for IPs were anti-mouse IgG or NKX3.1 and/or anti-β-catenin. The IPs and the blots were performed at least three times.