DMSO improves E-cadherin interaction with PIPKIγ, β- and p120-catenins.

<p>CHO cells transduced with the empty vector (Mock) or with WT, R749W or E757K hEcadherin were treated with 2% DMSO or with Medium, and the interaction of E-cadherin with PIPKIγ (A), p120- (B) and β-catenins (C) was assessed by PLA. Cells were fixed and incubated with antibodies against E-cadherin and PIPKIγ or E-cadherin and p120 or E-cadherin and β-catenin. In the negative control, CHO WT cells were incubated only with the antibody against E-cadherin. Close proximity of oligonucleotide-ligated secondary antibodies allows a rolling-circle amplification and the detection of the rolling-circle amplification product by a fluorescently labelled probe. Nuclei were counterstained with DAPI. The pictures were taken under a 40× objective. The number of spots per cell was quantified in each condition. The graphs show the average of relative number of blobs per cell + SE, n = 3 (* represents p≤0.05).