Adipose tissue inflammation in JNK1-deficient chimeras.

<p>Photomicrographs of adipose tissue sections were generated after staining with hematoxylin/eosin or anti-F4/80 and hematoxylin. Epididymal adipose tissue sections were prepared from all groups of mice, <i>Jnk1^−/−</i>→<i>WT, WT→WT, WT</i>→<i>Jnk1^−/−</i> and <i>Jnk1^−/−</i>→<i>Jnk1^−/−</i>, on high-fat diet for 27 weeks (A). In the same adipose tissue samples, total RNA was extracted and F4/80 mRNA expression level was quantified as an indicator of macrophage infiltration (B). Adipose tissue sections were also stained with anti-F4/80 antibody to detect the protein levels (C) and the number of crown like structures (CLS) were quantified (D). Expression levels of IL-1β, MCP-1, MIF-1, CD68, TNF-α, and IL-6 mRNAs in the epididymal adipose tissue were quantified to determine the cellular milieu in the WT (E) or <i>Jnk1^−/−</i> (F) recipient groups transplanted with <i>WT</i> or <i>Jnk1^−/−</i> bone marrow. Asterisk indicates statistical significance (<i>p</i><0.05) in Student's <i>t</i> test.