Histone acetylation correlates with NF-Y recruitment onto its target promoters.

<p>(A) ChIPs performed on proliferating (P) and terminally differentiated (TD) C2C12 cells using the indicated antibodies. No antibody was used as control (No Ab). (B) Q-ChIP analysis on proliferating (P) and terminally differentiated (TD) primary myoblasts, performed with the indicated antibodies. The promoters analyzed were: 1) <i>CyclinA2,</i> 2) <i>CyclinB1</i>, 3) <i>CyclinB2,</i> 4) <i>Cdk1</i>, 5) <i>Cdc25C,</i> 6) <i>Dhfr,</i> 7) <i>TopoisomeraseIIα,</i> 8) <i>Tk.</i> (C) Q-ChIP analysis of the <i>Myogenin</i> and <i>MLC</i> promoters performed with the indicated antibodies on proliferating (P) and terminally differentiated (TD) primary myoblasts. On B and C the enrichment of immunoprecipitated promoter fragments relative to the input was done. Rabbit IgG was used as control. (D) RT-PCR amplification of indicated genes was performed on proliferating (P) and terminal differentiated (TD) C2C12.