Histone acetylation modification at MHV-68 RTA promoter.

<p>(A) ChIP analysis of histone H3 acetylation level at MHV-68 RTA promoter. S11E cells were stimulated with TSA (200 ng/ml) for 4 hrs or mock-treated. Chromatin fragments were immunoprecipitated with an antibody against acetylated histones H3 (or no antibody control). Primers specific for RTA promoter or the GAPDH coding sequence were used to amplify the DNA isolated from the immunoprecipitated chromatin. The input DNAs and their dilutions were amplified to serve as positive controls. (B) ChIP analysis of histone H4 acetylation level at MHV-68 RTA promoter. Experiments were conducted as above, except an antibody against acetylated histone H4 was used. (C) HDAC3 was recruited to the MHV-68 RTA promoter. Recruitment of HDAC3, 5 or 7 to MHV-68 RTA promoter in S11E cells was investigated by ChIP. ChIP analysis of GAPDH coding sequence region is shown in the lower panel. Negative control (“No antibody”) and positive control (“Input”) were indicated. (D) ChIP analysis of HDAC3, acetylated histones H3 or acetylated histones H4 at RTA promoter. S11E cells were treated with 5-AzaC (10 µM, 36 hrs), TSA (200 ng/ml, 4 hrs) or left untreated, chromatin fragments immunoprecipitated and then analyzed by ChIP.