NIK is localized on the promoters of pro-inflammatory genes by CSE and TNFα leading to histone H3 acetylation in H292 cells.

<p>(A) Chromatin immunoprecipitation analysis of pro-inflammatory genes, <i>IL-6</i>, <i>IL-8, and COX-2</i> promoters with NIK antibody in H292 cells. Input DNA control is shown in the bottom panel. (B) NIK knockdown inhibited recruitment of NIK, and acetylated histone H3 to the promoters of pro-inflammatory genes in H292 cells in response to CSE. Images shown are representative of at least two separate experiments. After densitometry analysis, the values were normalized against the loading control, input. Data shown as mean ± S.E. (n = 2–3 per group). **, p<0.01, ***, p<0.001, significant compared with control group; #, p<0.05, ##, p<0.01, ###, p<0.001, significant compared with CSE-exposed group.