Histone acetylation modifications in co-cultured MSCs treated with NaB.

<p>MSCs were co-cultured with SMCs in a transwell chamber for 48 h. The MSCs were pretreated with 1.0 mM NaB before co-culturing. The MSCs in the co-culture system were harvested, and the genomic DNA was isolated and sonicated for a ChIP assay with antibodies against acetyl-histone H3K9, acetyl-histone H4 and normal rat IgG. The specific DNA fragments retrieved in the pull-down were further used for qPCR assays. The qPCR primers were designed to target the promoter of each gene. Values were given as folds of enrichment relative to the IgG control. Data are expressed as the mean ± SD of three biological replicates.*, <i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared to the untreated MSCs. ^#, <i>P</i><0.05, ^##<i>P</i><0.01, ^###<i>P</i><0.001 compared to the co-cultured MSCs.