Gating parameters of WT and mutant Kv7.1 channels expressed in the presence of WT KCNE1.

<p>V₅₀ (half activation voltage) and z (equivalent gating charge) were derived from fitting single Boltzmann function; I₆₀ corresponds to the current density measured at +60 mV in pA/pF. ΔG₀ and ΔΔG₀^c were calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001943#s4" target="_blank">methods</a>. Data are expressed as mean ± SEM and in parentheses are indicated the number of cells.^*, p<0.05 compared to WT (two-tailed, Student's unpaired t test). ND, not determined; NA, not applicable as R231W mutant is a constitutively open K⁺ leak channel.