Sirt1 KO Mice Have a Lower Level of Insulin

<div><p>(A) Plasma insulin levels in wild-type (open bars) or Sirt1 KO mice (black bars) <i>ad libitum</i> or after O/N starvation (<i>n</i> = 12 wild-type, 11 KO, *<i>p</i> < 0.03 in <i>ad libitum</i> and O/N starvation mice, ANOVA).</p> <p>(B) Plasma insulin levels in Sirt1 KO mice (black bar) compared with wild-type mice (open bars) 2, 10, or 20 min after injection with glucose (<i>n</i> = 4 or 5, *<i>p</i> < 0.05 compared with wild-type, ANOVA).</p> <p>(C) Insulin secretion in islets isolated from wild-type (open bars) or Sirt1 KO mice (black bars) after induction by 20 mM glucose for 1 h (<i>n</i> = 4, *<i>p</i> < 0.005 in wild-type, ANOVA).</p> <p>(D) Glucose levels in wild-type (open bars) and Sirt1 KO (black bars) mice (<i>n</i> = 12 wild-type, 11 KO, *<i>p</i> < 0.03 <i>ad libitum,</i> ANOVA).</p> <p>(E) Glucose tolerance tests in wild-type (black) and Sirt1 KO (green) mice (<i>n</i> = 6, *<i>p</i> < 0.05 at 20, 40, 60, and 120 min).</p>