Dectin-1 Dependent Cytokine and Chemokine Production in Response to <i>A. fumigatus</i>

<div><p>(A) Native RAW 264.7 macrophages or RAW 264.7 over-expressing murine dectin-1 were pretreated with isotype or 2A11 and co-cultured for 24 h with live <i>A. fumigatus.</i> Supernatant cytokine and chemokine levels were determined by Bio-Plex or ELISA. (A) illustrates cumulative results from five separate experiments. Asterisks, double asterisks, and the hash sign represent significant differences between RAW and RAW + 2A11, RAW and RAW-Dect, and RAW-Dect and RAW-Dect + 2A11, respectively (<i>p</i> < 0.05). Data are expressed as mean pg/ml + SEM.</p><p>(B–D) Identical experimental design with alveolar macrophages. (B–D) illustrate cumulative results from five separate experiments. Asterisks represent significant differences between isotype and 2A11 (<i>p</i> < 0.05). Data are expressed as mean pg/ml + SEM.</p><p>(E) Alveolar macrophages were pre-treated with 10-μM cytochalasin D and co-cultured with live <i>A. fumigatus</i> for 16 h. Supernatant cytokine and chemokine levels were determined by Bio-Plex or ELISA. (E) illustrates cumulative results from three separate experiments. Data are expressed as mean pg/ml + SEM.</p>