Internalisation and Dectin-1 protect murine pulmonary epithelia from <i>A. fumigatus</i>-mediated damage.

<p><b>(A)</b> Histopathology of Dectin-1^−/− and Dectin-1^+/+ leukopenic mice (n = 3) infected with CEA10 (10⁸ spores, 24 hr of infection, GMS and light green staining, 200× magnification). <b>(B)</b> LDH/BCA fold change in BALs from Dectin-1^−/− and Dectin-1^+/+ leukopenic mice (n = 3) infected as in B, technical triplicates. <b>(C)</b> S100B western blotting on lung homogenates from Dectin-1^−/− and Dectin-1^+/+ leukopenic mice infected as in B. Densitometry plot where pooled densitometry values for S100B were normalised on pooled densitometry values for actin and background subtracted for the saline controls. <b>(D)</b> Macrophages and neutrophils (cells/ml) in BALs from immunocompetent mice infected with <i>ΔpacC</i>^CEA10 mutant and CEA10 (n = 4, 10⁶ spores, 24 hr of infection). B and C: unpaired <i>t</i> test, *** <i>p</i><0.001 and * 0.01<<i>p</i><0.05.