Dectin-1 expression depends on PPARγ activation by IL-13 or PPARγ-specific ligands.

<p>(A) The surface protein level of Dectin-1 on peritoneal macrophages was measured by flow cytometry after treatment with IL-13 (50 ng/mL), rosiglitazone (RZ) (5 µM), 15d-PGJ2 (1 µM), MCC555 (5 µM) or GW1929 (1 µM). The changes in Dectin-1 induction were normalized to the untreated control value. Data are the means±SE of three separate experiments. (B) Representative FACS profiles of Dectin-1 (filled histograms) and isotype control labeling (unfilled histograms) in treated macrophages. Representative Dectin-1 FACS profiles of untreated macrophages (unfilled histograms) and treated (filled histograms) macrophages. (C) The mRNA level of Dectin-1 on peritoneal macrophages was quantified by quantitative real-time RT-PCR after treatment with IL-13 (50 ng/mL), rosiglitazone (RZ) (5 µM) or 15d-PGJ2 (1 µM). Data are the means±SE of three separate experiments. ** (p<0.01) and * (p<0.05) indicates a significant difference compared with the untreated macrophages. (D) The protein level of Dectin-1 on the murine cell line RAW264.7 transiently transfected with pCMV-luciferase (CMV-luc) or with pCMV-mPPARγ (CMV-PPARγ) and after treatment with IL-13 or rosiglitazone (RZ). Representative Dectin-1 FACS profiles of untreated (unfilled histograms) and treated (filled histograms) macrophages were obtained by flow cytometry. The changes in Dectin-1 induction were normalized to the untreated RAW 264.7 cells transfected with pCMV-luc. Data are the means±SE of three separate experiments. ** (p<0.01) and * (p<0.05) indicates a significant difference compared with the untreated RAW 264.7 cells transfected with pCMV-luc.