cPLA2 is involved in Dectin-1 induction by IL-13.

<p>(A) The Dectin-1 mRNA level of peritoneal macrophages was measured by quantitative real-time RT-PCR after treatment of peritoneal macrophages by an irreversible cPLA2 antagonist (MAFP) and by IL-13. Data are the means±SE of three separate experiments. (B) The protein level of Dectin-1 was measured by flow cytometry on peritoneal macrophages after treatment with MAFP (10 µM and 20 µM) and with IL-13 (50 ng/mL). Data are the means±SE of three separate experiments. (C) The protein level of Dectin-1 was measured by flow cytometry on peritoneal macrophages after treatment with MAFP and IL-13 and/or 15d-PGJ2 (1 µM). Data are the means±SE of three separate experiments. ** (p<0.01) and * (p<0.05) indicates a significant difference compared with the IL-13-treated macrophages.