Viral distribution in the tissues and organs of EV71-infected mice.

<p>Seven-day-old hSCARB2-Tg or non-Tg mice were injected with 3×10⁴ pfu of 5746 s.c. and on Day 7 post-viral infection, RNA was extracted from the brainstem (<b>A</b>), muscle (<b>B</b>), intestine (<b>C</b>), spinal cord (<b>D</b>), spleen (<b>E</b>), and skin (<b>F</b>) of the mice and then subjected to quantitative RT-PCR analysis using primers specific to the VP1 region. β-Actin gene expression in each tissue was used as the internal control. A schematic representation of the VP1 gene expression and the statistical average from 7 mice per group is shown. (<b>G</b>) Peripheral blood was collected from the individual mice on Day 2, Day 4, Day 6, and Day 8 post-infection and then subjected to an immune plaque assay described in the Materials and Methods section. The results are presented as the individual number of plaques, and the calculated mean in 100 µl of blood was obtained from 5 mice per group. Unpaired student <i>t</i> test with Welch’s correction was used for statistical analysis.