Expression of viral and inflammatory host genes in brain tissue collected from <i>Ptprc^L3X/+</i> and <i>Ptprc^L3X</i> infected mice.

<p>(<b>A</b>) <i>Ptprc^L3X/+</i> and <i>Ptprc^L3X</i> mice were infected i.p. with 1×10⁴ pfu of HSV-1. At the indicated day (D3, D6 and D10), total brains were harvested and mechanically homogenized for a plaque assay (n≥3). Viral titers are presented as pfu/gram of brain. The dotted line indicates the threshold of detection. (<b>B and C</b>) <i>Ptprc^L3X/+</i> and <i>Ptprc^L3X</i> mice were infected i.p. with 1×10⁴ pfu of HSV-1 (n = 9). Following infection, these mice were weighed two times daily. The brain stems of <i>Ptprc^L3X</i> mice that had lost at least 15% of their pre-infection weight were harvested. <i>Ptprc^L3X/+</i> mice were sacrificed and their brain stems were collected at days 7, 9, and 11 p.i. (n = 3 for each time point). The expression of the <i>ICP4</i> viral gene (<b>B</b>) and the indicated cellular genes (<b>C, upper panels</b>) was normalized to that of <i>hprt</i>. Data are presented as a fold increase relative to infected B6 samples. *, p-value (p)<0.05 and **, p<0.005. Correlations of expression levels were determined by comparing <i>ICP4</i> and the indicated cellular genes (<b>C, lower panels</b>).