Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4+ T cells stimulated with MmmSC Ags.

Gating strategy based on ISHAGE guidelines.

Patricia D. A. Lima (707898)
Zhilin Chen (291363)
Aysha Tayab (3826015)
Malia S. Q. Murphy (800163)
Jessica Pudwell (3826012)
Graeme N. Smith (627562)
B. Anne Croy (109241)

March 2017

Total CD45+ cells were gated, followed by Side Scatter (SS) vs CD34-FITC. The CD34+CD45+SS...

Representative dot-plots showing co-expression measured by FACS.

Natalia Sumption (781426)
Dudley T. Goodhead (781427)
Rhona M. Anderson (781428)

August 2015

Mononuclear cell sub-populations were gated and representative dot-plots produced showing levels ...

Absence of S1PL leads to accumulation of mature medullary thymocytes.

Peter Vogel (238749)
Michael S. Donoviel (375288)
Robert Read (375290)
Gwenn M. Hansen (304218)
Jill Hazlewood (375292)
Stephen J. Anderson (238778)
Weimei Sun (7276)
Jonathan Swaffield (375294)
Tamas Oravecz (238781)

February 2013

(A) Representative FACS histograms of expression of the CD69 and CD62L markers on S1PL+/+</s...

Expression of Activation Markers using flow cytometry assays.

Sieberth Nascimento-Brito (800928)
Jean Paulo Zukurov (505532)
Juliana T. Maricato (295063)
Angela C. Volpini (800929)
Anna Christina M. Salim (800930)
Flávio M. G. Araújo (800931)
Roney S. Coimbra (128325)
Guilherme C. Oliveira (224359)
Fernando Antoneli (800932)
Luiz Mário R. Janini (800933)

September 2015

Histograms showing the expression of CD25, CD69, CD38 and HLA-DR from gated CD3+CD4+ cell populat...

Both CD62L+CD4+ and CD62L−CD4+ T cells proliferate in response to MmmSC stimulation in vitro.

Philippe Totte (383309)
Aboubakar Yaya (383310)
Amadou Sery (383311)
Hezron Wesonga (383312)
Abel Wade (383313)
Jan Naessens (242148)
Mamadou Niang (383314)
François Thiaucourt (130211)

March 2013

Results of a typical four-color flow cytometric analysis are shown for one vaccinated animal. Cel...

Percentages of B cells, T cells, and DCs in the peripheral blood, spleen, and thymus of RasGRP4-null and WT B6 mice.

Saijun Zhou (3177975)
Kumiko Tanaka (553922)
Meredith O’Keeffe (3177969)
Miao Qi (665154)
Fatima El-Assaad (539925)
James C. Weaver (1775959)
Gang Chen (107840)
Christopher Weatherall (3177981)
Ying Wang (11406)
Bill Giannakopoulos (789111)
Liming Chen (113761)
DeMint Yu (3177972)
Matthew J. Hamilton (3177966)
Lislaine A. Wensing (3177978)
Richard L. Stevens (303328)
Steven A. Krilis (789110)

March 2016

Flow cytometry was performed on the peripheral blood cells, splenocytes, and thymocytes from RasG...

Typical flow cytometric data for lymphocyte, CD4+ memory, and CD4+ naive cell populations.

Nels C. Olson (449941)
Margaret F. Doyle (449942)
Nancy Swords Jenny (239523)
Sally A. Huber (449943)
Bruce M. Psaty (103721)
Richard A. Kronmal (449944)
Russell P. Tracy (127552)

August 2013

At least 30,000 lymphocytes were evaluated and were gated based on their forward (FCS; X-axis) an...

A representative example of surface antigen expression.

Steffanie Sabbaj (337868)
Zdenek Hel (337869)
Holly E. Richter (337870)
Jiri Mestecky (337871)
Paul A. Goepfert (50345)

February 2013

Cell surface expression of CD62L, HLA-DR and αEβ7 on A. CD3+CD4+ and B. CD3+CD8+ T ...

Surface expression of co-stimulatory molecules by DCs in response to S. suis.

Marie-Pier Lecours (135584)
Mariela Segura (135590)
Nahuel Fittipaldi (135592)
Serge Rivest (135596)
Marcelo Gottschalk (135599)

September 2012

WT, MyD88−/−, and TLR2−/− DCs (106 cells/ml) were stimulated wit...

Flow cytometry gating strategy for B220-positive B lymphocytes.

Daichi Akasaka (14670684)
Satoru Iguchi (14670687)
Ryusuke Kaneko (14670690)
Yohei Yoshiga (111997)
Daisuke Kajiwara (14670693)
Yoshinori Nakachi (14670696)
Naruto Noma (14670699)
Kenji Tanaka (692662)
Atsushi Shimizu (1862)
Fumihito Hosoi (2538544)

February 2023

Total lymphocytes were initially gated on an FSC versus SSC plot (left) and then gated on the CD45R/...

Cell surface marker expression in DmpCre/Ai9+ and DmpCre/Ai9− populations of BOC cultures.

Elena Torreggiani (455391)
Brya G. Matthews (455392)
Slavica Pejda (455393)
Igor Matic (455394)
Mark C. Horowitz (455395)
Danka Grcevic (455396)
Ivo Kalajzic (455397)

September 2013

Primary BOC isolated from Dmp1Cre/Ai9 mice were grown for ten days before analysis. Nontransgenic...

Sustained Tl1a expression leads to an increased percentage of activated T cells.

David Q. Shih (357858)
Robert Barrett (208665)
Xiaolan Zhang (76101)
Nicole Yeager (357859)
Hon Wai Koon (339765)
Piangwarin Phaosawasdi (357860)
Yahui Song (357861)
Brian Ko (357862)
Michelle H. Wong (241000)
Kathrin S. Michelsen (241001)
Gislaine Martins (357864)
Charalabos Pothoulakis (357866)
Stephan R. Targan (357868)

February 2013

FACS plot of 2 month (A) and 10 month (B) splenocytes and MLN cells showing expression of activat...

MDM2-specific CD8+ T cells are present in the periphery of Agpos mice and express reduced levels of TCR and CD8 but high levels of CD44.

Gavin M. Bendle (379048)
Shao-An Xue (379050)
Angelika Holler (379052)
Hans J. Stauss (379054)

February 2013

(A) Lymph nodes cells from 6–8 week old Agpos and Agneg mice were stained w...

Upregulation of both TLR2 and TLR4 on the surface of mouse bone marrow-derived DCs stimulated by rTs-Hsp70.

Rui Zhang (13940)
Qing Sun (492552)
Yi Chen (38576)
Ximeng Sun (710371)
Yuan Gu (348663)
Zhang Zhao (5236604)
Yuli Cheng (116606)
Limei Zhao (289815)
Jingjing Huang (170112)
Bin Zhan (11041)
Xinping Zhu (297790)

May 2018

Representative dot plots for the gating strategy: (I) gating on viable cells, (II) selection of n...

Immunophenotypes of ex-vivo expanded retinal-induced Tregs.

Jung Ho Lee (441202)
Eun-Joo Jeon (441203)
Nayoun Kim (441204)
Young-Sun Nam (441205)
Keon-Il Im (441206)
Jung-Yeon Lim (441207)
Eun-Jung Kim (278998)
Mi-La Cho (46882)
Ki Taik Han (441208)
Seok-Goo Cho (424174)

August 2013

(A) Retinal-induced CD4+CD25+ Tregs showed >96% purity on flow cytometry. (...

Gating strategy based on ISHAGE guidelines.

Patricia D. A. Lima (707898)
Zhilin Chen (291363)
Aysha Tayab (3826015)
Malia S. Q. Murphy (800163)
Jessica Pudwell (3826012)
Graeme N. Smith (627562)
B. Anne Croy (109241)

March 2017

Total CD45+ cells were gated, followed by Side Scatter (SS) vs CD34-FITC. The CD34+CD45+SS...

Representative dot-plots showing co-expression measured by FACS.

Natalia Sumption (781426)
Dudley T. Goodhead (781427)
Rhona M. Anderson (781428)

August 2015

Mononuclear cell sub-populations were gated and representative dot-plots produced showing levels ...

Absence of S1PL leads to accumulation of mature medullary thymocytes.

Peter Vogel (238749)
Michael S. Donoviel (375288)
Robert Read (375290)
Gwenn M. Hansen (304218)
Jill Hazlewood (375292)
Stephen J. Anderson (238778)
Weimei Sun (7276)
Jonathan Swaffield (375294)
Tamas Oravecz (238781)

February 2013

(A) Representative FACS histograms of expression of the CD69 and CD62L markers on S1PL+/+</s...

Expression of Activation Markers using flow cytometry assays.

Sieberth Nascimento-Brito (800928)
Jean Paulo Zukurov (505532)
Juliana T. Maricato (295063)
Angela C. Volpini (800929)
Anna Christina M. Salim (800930)
Flávio M. G. Araújo (800931)
Roney S. Coimbra (128325)
Guilherme C. Oliveira (224359)
Fernando Antoneli (800932)
Luiz Mário R. Janini (800933)

September 2015

Histograms showing the expression of CD25, CD69, CD38 and HLA-DR from gated CD3+CD4+ cell populat...

Both CD62L+CD4+ and CD62L−CD4+ T cells proliferate in response to MmmSC stimulation in vitro.

Philippe Totte (383309)
Aboubakar Yaya (383310)
Amadou Sery (383311)
Hezron Wesonga (383312)
Abel Wade (383313)
Jan Naessens (242148)
Mamadou Niang (383314)
François Thiaucourt (130211)

March 2013

Results of a typical four-color flow cytometric analysis are shown for one vaccinated animal. Cel...

Percentages of B cells, T cells, and DCs in the peripheral blood, spleen, and thymus of RasGRP4-null and WT B6 mice.

Saijun Zhou (3177975)
Kumiko Tanaka (553922)
Meredith O’Keeffe (3177969)
Miao Qi (665154)
Fatima El-Assaad (539925)
James C. Weaver (1775959)
Gang Chen (107840)
Christopher Weatherall (3177981)
Ying Wang (11406)
Bill Giannakopoulos (789111)
Liming Chen (113761)
DeMint Yu (3177972)
Matthew J. Hamilton (3177966)
Lislaine A. Wensing (3177978)
Richard L. Stevens (303328)
Steven A. Krilis (789110)

March 2016

Flow cytometry was performed on the peripheral blood cells, splenocytes, and thymocytes from RasG...

Typical flow cytometric data for lymphocyte, CD4+ memory, and CD4+ naive cell populations.

Nels C. Olson (449941)
Margaret F. Doyle (449942)
Nancy Swords Jenny (239523)
Sally A. Huber (449943)
Bruce M. Psaty (103721)
Richard A. Kronmal (449944)
Russell P. Tracy (127552)

August 2013

At least 30,000 lymphocytes were evaluated and were gated based on their forward (FCS; X-axis) an...

A representative example of surface antigen expression.

Steffanie Sabbaj (337868)
Zdenek Hel (337869)
Holly E. Richter (337870)
Jiri Mestecky (337871)
Paul A. Goepfert (50345)

February 2013

Cell surface expression of CD62L, HLA-DR and αEβ7 on A. CD3+CD4+ and B. CD3+CD8+ T ...

Surface expression of co-stimulatory molecules by DCs in response to S. suis.

Marie-Pier Lecours (135584)
Mariela Segura (135590)
Nahuel Fittipaldi (135592)
Serge Rivest (135596)
Marcelo Gottschalk (135599)

September 2012

WT, MyD88−/−, and TLR2−/− DCs (106 cells/ml) were stimulated wit...

Flow cytometry gating strategy for B220-positive B lymphocytes.

Daichi Akasaka (14670684)
Satoru Iguchi (14670687)
Ryusuke Kaneko (14670690)
Yohei Yoshiga (111997)
Daisuke Kajiwara (14670693)
Yoshinori Nakachi (14670696)
Naruto Noma (14670699)
Kenji Tanaka (692662)
Atsushi Shimizu (1862)
Fumihito Hosoi (2538544)

February 2023

Total lymphocytes were initially gated on an FSC versus SSC plot (left) and then gated on the CD45R/...

Cell surface marker expression in DmpCre/Ai9+ and DmpCre/Ai9− populations of BOC cultures.

Elena Torreggiani (455391)
Brya G. Matthews (455392)
Slavica Pejda (455393)
Igor Matic (455394)
Mark C. Horowitz (455395)
Danka Grcevic (455396)
Ivo Kalajzic (455397)

September 2013

Primary BOC isolated from Dmp1Cre/Ai9 mice were grown for ten days before analysis. Nontransgenic...

Sustained Tl1a expression leads to an increased percentage of activated T cells.

David Q. Shih (357858)
Robert Barrett (208665)
Xiaolan Zhang (76101)
Nicole Yeager (357859)
Hon Wai Koon (339765)
Piangwarin Phaosawasdi (357860)
Yahui Song (357861)
Brian Ko (357862)
Michelle H. Wong (241000)
Kathrin S. Michelsen (241001)
Gislaine Martins (357864)
Charalabos Pothoulakis (357866)
Stephan R. Targan (357868)

February 2013

FACS plot of 2 month (A) and 10 month (B) splenocytes and MLN cells showing expression of activat...

MDM2-specific CD8+ T cells are present in the periphery of Agpos mice and express reduced levels of TCR and CD8 but high levels of CD44.

Gavin M. Bendle (379048)
Shao-An Xue (379050)
Angelika Holler (379052)
Hans J. Stauss (379054)

February 2013

(A) Lymph nodes cells from 6–8 week old Agpos and Agneg mice were stained w...

Upregulation of both TLR2 and TLR4 on the surface of mouse bone marrow-derived DCs stimulated by rTs-Hsp70.

Rui Zhang (13940)
Qing Sun (492552)
Yi Chen (38576)
Ximeng Sun (710371)
Yuan Gu (348663)
Zhang Zhao (5236604)
Yuli Cheng (116606)
Limei Zhao (289815)
Jingjing Huang (170112)
Bin Zhan (11041)
Xinping Zhu (297790)

May 2018

Representative dot plots for the gating strategy: (I) gating on viable cells, (II) selection of n...

Immunophenotypes of ex-vivo expanded retinal-induced Tregs.

Jung Ho Lee (441202)
Eun-Joo Jeon (441203)
Nayoun Kim (441204)
Young-Sun Nam (441205)
Keon-Il Im (441206)
Jung-Yeon Lim (441207)
Eun-Jung Kim (278998)
Mi-La Cho (46882)
Ki Taik Han (441208)
Seok-Goo Cho (424174)

August 2013

(A) Retinal-induced CD4+CD25+ Tregs showed >96% purity on flow cytometry. (...

Gating strategy based on ISHAGE guidelines.

Patricia D. A. Lima (707898)
Zhilin Chen (291363)
Aysha Tayab (3826015)
Malia S. Q. Murphy (800163)
Jessica Pudwell (3826012)
Graeme N. Smith (627562)
B. Anne Croy (109241)

March 2017

Total CD45+ cells were gated, followed by Side Scatter (SS) vs CD34-FITC. The CD34+CD45+SS...

Representative dot-plots showing co-expression measured by FACS.

Natalia Sumption (781426)
Dudley T. Goodhead (781427)
Rhona M. Anderson (781428)

August 2015

Mononuclear cell sub-populations were gated and representative dot-plots produced showing levels ...

Absence of S1PL leads to accumulation of mature medullary thymocytes.

Peter Vogel (238749)
Michael S. Donoviel (375288)
Robert Read (375290)
Gwenn M. Hansen (304218)
Jill Hazlewood (375292)
Stephen J. Anderson (238778)
Weimei Sun (7276)
Jonathan Swaffield (375294)
Tamas Oravecz (238781)

February 2013

(A) Representative FACS histograms of expression of the CD69 and CD62L markers on S1PL+/+</s...

Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4<sup>+</sup> T cells stimulated with <i>Mmm</i>SC Ags.

Abstract

Extracted data

Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4<sup>+</sup> T cells stimulated with <i>Mmm</i>SC Ags.

Abstract

Extracted data

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