MDM2-specific CD8⁺ T cells are present in the periphery of Ag^pos mice and express reduced levels of TCR and CD8 but high levels of CD44.

<p>(A) Lymph nodes cells from 6–8 week old Ag^pos and Ag^neg mice were stained with antibodies against CD4, CD8 and Vβ7 and analysed by flow cytometry. Numbers in quadrants are percentage of live-gated lymphocytes. (B) Lymph node cells were stained with antibodies against CD8, Vβ7 and the activation markers CD44, CD62L, CD69 and CD25. Histograms display gated viable CD8⁺Vβ7⁺ cells. Numbers in histograms represent the specific MFI of the activation marker staining for the gated population. Data are representative of at least three independent experiments.