Gating strategy based on ISHAGE guidelines.
- Patricia D. A. Lima (707898)
- Zhilin Chen (291363)
- Aysha Tayab (3826015)
- Malia S. Q. Murphy (800163)
- Jessica Pudwell (3826012)
- Graeme N. Smith (627562)
- B. Anne Croy (109241)
Publication date
March 2017
DOIAbstract
<p>Total CD45+ cells were gated, followed by Side Scatter (SS) <i>vs</i> CD34-FITC. The CD34+CD45+SSlow subset was selected (Gate J). A second SS <i>vs</i> CD45-PE-Cy7 gating was performed to select CD45dim cells (Gate M), and confirmed by a low Forward Scatter (R- a shared lymphocyte gate; Gate O). Analyzing CD34-FITC <i>vs</i> CD45-PE-Cy7 cells, a subset should be coincident with gate Q (lymphocyte). Expression of CD133 and/or KDR was assessed on CD34+CD45dimSSlow cells (gated O).</p
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