Gating strategy for mononuclear cell expression.
- Olamide D. Jarrett (756178)
- Kirsten E. Brady (334255)
- Sharada P. Modur (499120)
- Jill Plants (509657)
- Alan L. Landay (231177)
- Mahmood Ghassemi (61122)
- Elizabeth T. Golub (403491)
- Greg T. Spear (313861)
- Richard M. Novak (287233)
Publication date
June 2015
DOIAbstract
<p>Dead cells were excluded with a viability stain before gating on CD45+ cells. CD45+ cells were further analyzed for CD3+ and CD14+ expression. CD45+CD3+ cells were gated on CD4+ or CD8+ before HLA-DR/CD38 quadrant gating as well as CCR5 co-receptor expression. CD45+CD14+ cells were also analyzed for CCR5 expression.</p
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