Inhibition of miR-21 increases cell apoptosis of arsenite-transformed HELF cells.

<p>Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. Arsenite-transformed HELF cells were transfected with 150 nM of anti-miR-nc or anti-miR-21 for 24 h. (A) Western blots and (B) relative protein levels of cleaved caspase-3 and caspase-3 (means ± SD, n = 3). **<i>P</i><0.01 different from NC group. (C) The rate of cell apoptosis was analyzed by the Hoechst 33258 assay (means ± SD, n = 3). **<i>P</i><0.01 different from NC group.