ERK and JNK are involved in the regulations of miR-21 to protein levels of Pdcd4 and Spry1 and to cell apoptosis in arsenite-transformed HELF cells.

<p>Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. Arsenite-transformed HELF cells were treated with 10 µM of LY294002 (an inhibitor of PI-3K), SP600125 (an inhibitor of JNK), or U0126 (an inhibitor of ERK) for 24 h. (A) Western blots and (B) relative protein levels (means ± SD, n = 3) of p-Akt, p-ERK, and p-JNK. (C) qRT-PCR analysis of miR-21 expression (means ± SD, n = 3). **<i>P</i><0.01 difference from control group. (D) Western blots (upper) and RT-PCR (below) analyses for protein and mRNA levels and (E) relative protein levels (means ± SD, n = 3) of Pdcd4, Spry1, cleaved-caspase-3, and caspase-3. **<i>P</i><0.01 difference from control group.