Silencing of miR-214 induces apoptosis in NPC cells.

<p>(A) Forty-eight hours post-transfection, miR-214 expression levels (normalized to <i>U6 RNA</i>) were significantly decreased by 82% (<i>P</i><0.01) in CNE2/LNA-antimiR-214 and 87.7% (<i>P</i><0.01) in SUNE1/LNA-antimiR-214 cells, relative to the LNA-control. (B) Forty-eight hours post-transfection, Caspase 3 activity in the cells were measured by spectrophotometry. The relative activity of Caspase 3 was significantly increased in CNE2/LNA-antimiR-214 cells and SUNE1/LNA-antimiR-214 cells. (C) Forty-eight hours post-transfection, apoptosis was determined by flow cytometric detection of Annexin-V-FITC-positive/PI-negative cells. Bars are shown as the mean ± SD of cells with Annexin-V-FITC-positive and PI-negative. All data are shown as mean ± SD of triplicate experiments. ** <i>P</i><0.01.