p53 knockdown by its siRNA abolished the apoptotic effects of miR-214-transfection and COP1 knockdown.

<p>(A) 10 nM of newly designed siR-tp53 successfully knockdowned the expression of p53 protein from 24 hours up to 72 hours post-transfection in Ud6 cell line. (B) p53 knockdown by siR-tp53 abolished the effect of apoptotic induction in Ud6 cells. Co-transfection of Ud6 cells with miR-214 or siR-cop1 and control RNA induced apoptosis; however, when siR-tp53 replaced the control RNA, apoptosis was hardly or only slightly induced in the cells. Scale bars in the photographs indicate 100 μm. All final concentrations of small RNA transfection were 10 nM in this examination. All data are present as the mean of triplicate experiments with error bars indicating the s.d. The statistical significances stated were referred to the entire Annexin/PI population (Two-tailed t-test; *p<0.05, **p<0.01).