NF-κB and c-Jun are involved in the regulations of miR-21 to protein levels of Pdcd4 and Spry1 and to cell apoptosis in arsenite-transformed HELF cells.

<p>Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. Arsenite-transformed HELF cells were transfected with 50 nM of control siRNA, NF-κB p65 siRNA, or c-Jun siRNA for 24 h. (A) Western blots and (B) relative protein levels (means ± SD, n = 3) of NF-κB p65 and c-Jun. (C) qRT-PCR analysis of miR-21 expression (means ± SD, n = 3). **<i>P</i><0.01 difference from Con siRNA group. (D) Western blots (upper) and RT-PCR (below) analyses for protein and mRNA levels and (E) relative protein levels (means ± SD, n = 3) of Pdcd4, Spry1, cleaved-caspase-3, and caspase-3, **<i>P</i><0.01 difference from the control (Con) siRNA group.