qPCR analysis of the expression of 16 differently expressed genes identified through microarray analysis in different transgenic lines.

<p>(A) Confirmation of microarray data by examining 16 genes selected from the list of up- or down-regulated genes based on our microarray analysis using total RNA extracted from 3 months old <i>Tg-I</i> liver. (B–D) Analysis of the expression of these 16 genes in different transgenic lines at different ages. Gene expression was analyzed by qPCR using gene specific primers and was expressed as fold change after normalization against <i>elf1a</i>. The value of CK was set as 1. CK: wild type adult liver control. *: <i>p</i><0.05, **: <i>p</i><0.01.