High variability in the <i>MAT</i>-flanking region in <i>Z. rouxii</i>.

<p>(A) Gene organization around the <i>MAT</i>-like locus in <i>Z. rouxii</i> CBS732, which was used for genomic sequencing. Small arrows indicate primers. The vertical bars are telomeres. The box indicates a gene used for a probe in Fig. 2. (B) PCR amplification of the <i>MAT</i>-like locus from <i>Z. rouxii</i> CBS732, DA2, DL2, NBRC1130, ATCC2623, NBRC0686, NBRC0740, NBRC1053, and NBRC1733. (C) Arrangement of chromosomes. Partial chromosome structures around the <i>MAT</i>-like loci were reconstructed. Theoretically, all possible combinations of pieces to yield PCR amplicons detected in (B) are shown. Additionally, refer to our prediction model in Fig. 4. Coloring corresponds to gene position in CBS732: pink, left side of <i>HMR</i>; blue, right side of <i>HMR</i>; dark green, left side of <i>MAT</i>; orange, between <i>MAT</i> and <i>HML</i>; and wine red, right side of <i>HML</i>.