Schematic of MLPA probes designed for hotspot chromosome arms and MLPA data generated from GCR–containing isolates derived from a wild-type strain.

<p>Filled squares represent telomeres and filled circles represent centromeres. Hollow triangles represent delta sequences and pairs of hollow triangles connected by blue lines represent Ty1 or Ty2 sequences. The transcriptional orientation of the elements is represented by the direction of the triangles. Solid orange lines above the chromosome arms represent chromosome arm duplications predicted to be mediated by a Ty element; dotted green lines represent duplications predicted to be mediated by a non-Ty element. A. MLPA data for chromosome III-R. Hotspots exist at FS1 and FS2. FS1 represents a tandem pair of Tys replacing the sequence between the 5′ end of <i>SRD1</i> to <i>YCRWdelta10</i>. FS2 replaces <i>YCRWdelta11</i> with an inverted pair of Tys separated by a short spacer sequence. See Ref <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002539#pgen.1002539-Lemoine2" target="_blank">[15]</a>. B. MLPA data for chromosome V-R. Hotspots exist at or near <i>YERWdelta17</i>/<i>YERWdelta21</i>/<i>YERCTy1-1</i> and the YERCdelta14/<i>YERCdelta15</i>/<i>YERCdelta16</i> loci. C. MLPA data for chromosome IXV-R. A hotspot exists at the <i>YNLCTy2-1</i> Ty2 locus. D. MLPA data for chromosome X-R. A hotspot occurs at the <i>YJRWTy1-1/YJRWTy1-2</i> locus.