The structure of targeting vector (first line), wild type locus (second line), targeted allele after homologous recombination (third line) and allele after removal of the cassette by FLP recombinase (fourth line) is presented

<p><b>Copyright information:</b></p><p>Taken from "A new GFP-tagged line reveals unexpected Otx2 protein localization in retinal photoreceptors"</p><p>http://www.biomedcentral.com/1471-213X/7/122</p><p>BMC Developmental Biology 2007;7():122-122.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2204009.</p><p></p> Gray boxes are coding regions, whites boxes are 5' and 3' UTR regions, yellow box is selection cassette, green box is cDNA. Red triangles are sites. Bent arrows symbolize the three main transcription starts sites known for gene [19, 32]. Dotted lines show BamH I fragments detected by southern blot analysis using probe represented by a thick line. PCR primers used are indicated by arrows. Product obtained with A and B primers is shown. Scale bar and sizes of fragments are indicated. PCR analysis of NeoES clones using primers A and B showing two non-homologous (N) and one homologous (H) recombinants. Southern blot analysis using BamH I digested genomic DNA and probe indicated in of wild type and homologous recombinant clones. Genotypes are indicated. PCR genotyping of mice produced from ES cells. Analyse was done using C, D and E primers. Sizes, schematic representations of amplified fragments (see part for legend) and deduced genotypes are indicated