<p>Down-regulation of FRAT1 mRNA and protein expression in U251-S cells as compared to the parental U251, U251-NC, and U251-neo control cell lines was confirmed by RT-PCR and Western blot (WB). GAPDH was amplified as an internal control for the RT-PCR, and β-actin levels were examined as a loading control for the Western blot. 1: parental U251 cells; 2: U251-NC; 3: U251-neo; 4: U251-S.</p
<p>Western blotting analysis of p21<sup>Cip1</sup>, p27<sup>Kip1</sup>, cyclin D1 and FOXO3a protein...
<p>(A) U251, U251-C, and U251-UL23 cells were transfected with reporter plasmid pGL3-Promoter-3×GAS ...
<p>Shown is a representative result from three independent experiments with Western blotting. GAPDH ...
<p>Cell viability was measured using a MTT assay. Cell growth curves were determined by absorbance a...
<p><i>Left panel:</i> (A) parental U251, (B) U251-NC, (C) U251-neo and (D) U251-S were stained with ...
<p>MCF-7 cells were transfected with nonspecific control siRNA or siRNA sequence for E2A, FOXO1 or F...
stealth™ siRNAs (S#1, S#2, and S#3) by using nucleofection. Cell lysates were prepared 24 and 48 h a...
stealth™ siRNAs (S#1, S#2, and S#3) by using nucleofection. Cell lysates were prepared 24 and 48 h a...
<p>Immunoblots of protein extracts from cell lines after RNA interference. Panel A: eEF1Bα, eEF1Bδ a...
nucleofection. Cell lysates were prepared 24 and 48 h after transfection and mRNA and protein expres...
<p>(A) Western blots of protein extracts from A549 cells, HepG2 cells, A549 cells treated with contr...
<p>Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, ...
Background: FRAT1 positively regulates the Wnt/b-catenin signaling pathway by inhibiting GSK-3-media...
<p>Expression of miR-21 was showed in 4T1 cells (A) and HUVECs (B) that transfected with miR-21 mimi...
<p>Cell apoptosis in each group were detected using an RNA interference technique and AV/PI staining...
<p>Western blotting analysis of p21<sup>Cip1</sup>, p27<sup>Kip1</sup>, cyclin D1 and FOXO3a protein...
<p>(A) U251, U251-C, and U251-UL23 cells were transfected with reporter plasmid pGL3-Promoter-3×GAS ...
<p>Shown is a representative result from three independent experiments with Western blotting. GAPDH ...
<p>Cell viability was measured using a MTT assay. Cell growth curves were determined by absorbance a...
<p><i>Left panel:</i> (A) parental U251, (B) U251-NC, (C) U251-neo and (D) U251-S were stained with ...
<p>MCF-7 cells were transfected with nonspecific control siRNA or siRNA sequence for E2A, FOXO1 or F...
stealth™ siRNAs (S#1, S#2, and S#3) by using nucleofection. Cell lysates were prepared 24 and 48 h a...
stealth™ siRNAs (S#1, S#2, and S#3) by using nucleofection. Cell lysates were prepared 24 and 48 h a...
<p>Immunoblots of protein extracts from cell lines after RNA interference. Panel A: eEF1Bα, eEF1Bδ a...
nucleofection. Cell lysates were prepared 24 and 48 h after transfection and mRNA and protein expres...
<p>(A) Western blots of protein extracts from A549 cells, HepG2 cells, A549 cells treated with contr...
<p>Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, ...
Background: FRAT1 positively regulates the Wnt/b-catenin signaling pathway by inhibiting GSK-3-media...
<p>Expression of miR-21 was showed in 4T1 cells (A) and HUVECs (B) that transfected with miR-21 mimi...
<p>Cell apoptosis in each group were detected using an RNA interference technique and AV/PI staining...
<p>Western blotting analysis of p21<sup>Cip1</sup>, p27<sup>Kip1</sup>, cyclin D1 and FOXO3a protein...
<p>(A) U251, U251-C, and U251-UL23 cells were transfected with reporter plasmid pGL3-Promoter-3×GAS ...
<p>Shown is a representative result from three independent experiments with Western blotting. GAPDH ...