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Immunoblotting of STMN1 and IGF1R in Ca9-22 cells transfected with Pre-miR-223 and Pre-miR-NC.

Hirohiko Tachibana (3152577)
Ri Sho (738959)
Yuji Takeda (178444)
Xuhong Zhang (2046028)
Yukie Yoshida (550019)
Hiroto Narimatsu (738956)
Katsumi Otani (738960)
Shigeo Ishikawa (3152580)
Akira Fukao (478046)
Hironobu Asao (2604073)
Mitsuyoshi Iino (3152574)

Publication date

July 2016

DOI

10.1371/journal.pone.0159693.g005

Abstract

<p>Shown is a representative result from three independent experiments with Western blotting. GAPDH was used as a control.</p

Extracted data

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miR-103 expression analysis in IGF-1 stimulated mouse small intestinal crypt cells.

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IGF1R expression rescue from miR-223 suppression.

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<div><p><b>A</b>&<b>B</b>. The mRNA expression of IGF1R (A) and Sp1 (B) was monitored by qRT-PCR exa...

Molecular effects induced by LNA-i-miR-221 transfection in MM cells.

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<p>Transfected M23 and SP6.5 cells were prepared and used for Western blot analysis with multiple an...

Western blot showing IgG, RAG1 and RAG2 expression in sarcoma cells.

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IGF-1R was directly targeted by miR-223.

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February 2013

<p>(A) Quantitative PCR results of IGF-1R. Two pairs of primers (IGF-1R-1 and IGF-1R-2) designed at ...

Insulin-like growth factor 1 receptor (IGF-1R) is targeted by miR-223.

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February 2013

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Identification of the optimal anti-myostatin miRNAs in 293FT cells.

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Mingrui Huang (632418)
Feng Wang (44414)

September 2014

<p>Each of the four pD-miRNA plasmids was co-transfected with C1-MSTN into 293FT cells. Protein leve...

Higher expression of miR-351 modulates the expression of the nucleus intermediate filament lamin-B1.

Akira Sato (210501)
Takuya Omi (2608192)
Akihiro Yamamoto (2608189)
Akito Satake (2301061)
Akiko Hiramoto (2016034)
Mitsuko Masutani (78971)
Sei-ichi Tanuma (77409)
Yusuke Wataya (452256)
Hye-Sook Kim (452255)

April 2016

<p>(A) At 48 hours after transfection with the vehicle, non-silencing siRNA (NSsi) or the mature miR...

Effects of miR-137 on the IGFBP-5 expression in VSMCs.

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<p>(A) The relative mRNA expression levels of IGFBP-5 in VSMCs after miR-137 mimics, miR-137 inhibit...

miR-182 downregulates the expression of MITF and c-Met.

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Western blot showing NF-κB p65, E2A, FOXO1, FOXP1, RAG expression in epithelial cancer cells.

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Yuxuan Liu (349237)
Yingying Zhao (349236)
Changchun Ma (349239)
Jin Luo (349243)
Yamei Qiu (349241)
Guowei Huang (129576)
Christine Korteweg (126198)
Jiang Gu (126204)

February 2013

<p>Raji cell was used as a positive control. GAPDH was used as an internal control.</p

Reduction in MYCN protein levels in NB cells after treatment with small molecules.

Inga Müller (568256)
Karin Larsson (568257)
Anna Frenzel (194997)
Ganna Oliynyk (568258)
Hanna Zirath (195000)
Edward V. Prochownik (163628)
Nicholas J. Westwood (254891)
Marie Arsenian Henriksson (195006)

May 2014

<p>Western blot analysis of MYCN expression in SK-N-BE(2) cells treated for 48 hours with the indica...

HDAC4 and FGFBP1 expression levels in C2C12 cells transiently transfected with miRNA-206.

Valeria Valsecchi (494030)
Marina Boido (306249)
Elena De Amicis (494031)
Antonio Piras (347544)
Alessandro Vercelli (139469)

June 2015

<p>Panels A-B, representative Western blots displaying HDAC4 protein levels in C2C12 cells transfect...

miR-103 expression analysis in IGF-1 stimulated mouse small intestinal crypt cells.

Yalin Liao (361471)
Bo Lönnerdal (361472)

February 2013

<p>(A) microrarray analysis of total miRNA expression in crypt cells after stimulation with IGF-1 fo...

IGF1R expression rescue from miR-223 suppression.

Li Hui Wu (492959)
Qian Qian Cai (492960)
Yi Wei Dong (340604)
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Bing Qi (492962)
Chang Jun Xu (492963)
Xing Zhong Wu (340608)

December 2013

<div><p><b>A</b>&<b>B</b>. The mRNA expression of IGF1R (A) and Sp1 (B) was monitored by qRT-PCR exa...

Molecular effects induced by LNA-i-miR-221 transfection in MM cells.

Maria Teresa Di Martino (525637)
Annamaria Gullà (525638)
Maria Eugenia Gallo Cantafio (525639)
Emanuela Altomare (525640)
Nicola Amodio (525641)
Emanuela Leone (525642)
Eugenio Morelli (525643)
Santo Giovanni Lio (525644)
Daniele Caracciolo (525645)
Marco Rossi (109689)
Niels M. Frandsen (525646)
Pierosandro Tagliaferri (525647)
Pierfrancesco Tassone (525648)

February 2014

<p>miR-221(A) q-RT-PCR 24, 48 and 72 hours after transfection with LNA-i-miR-221 and LNA-i-miR-NC in...

miR-182 downregulates multiple cell signal pathways.

Dongsheng Yan (146499)
Xiang Da Dong (146502)
Xiaoyan Chen (146508)
Shasha Yao (146515)
Lihua Wang (14548)
Jiao Wang (47178)
Chao Wang (146527)
Dan-Ning Hu (146536)
Jia Qu (28271)
LiLi Tu (3560126)

July 2012

<p>Transfected M23 and SP6.5 cells were prepared and used for Western blot analysis with multiple an...

Western blot showing IgG, RAG1 and RAG2 expression in sarcoma cells.

Zhengshan Chen (126192)
Jing Li (10611)
Yanna Xiao (349235)
Junjun Zhang (2035)
Yingying Zhao (349236)
Yuxuan Liu (349237)
Changchun Ma (349239)
Yamei Qiu (349241)
Jin Luo (349243)
Guowei Huang (129576)
Christine Korteweg (126198)
Jiang Gu (126204)

February 2013

<p>A, IgG protein was expressed in sarcoma cells. FBS was used as a negative control and human IgG (...

IGF-1R was directly targeted by miR-223.

Cheng You Jia (340599)
Hui Hui Li (340602)
Xu Chao Zhu (340603)
Yi Wei Dong (340604)
Da Fu (114238)
Qian Lei Zhao (340606)
Wei Wu (27708)
Xing Zhong Wu (340608)

February 2013

<p>(A) Quantitative PCR results of IGF-1R. Two pairs of primers (IGF-1R-1 and IGF-1R-2) designed at ...

Insulin-like growth factor 1 receptor (IGF-1R) is targeted by miR-223.

Quan Wang (31224)
De-Yu Zhao (721771)
Hong Xu (71477)
Hui Zhou (13228)
Qian-Yuan Yang (721772)
Feng Liu (72874)
Guo-Ping Zhou (312844)

April 2015

<p>(A and B) The cells were transfected with miR-223 mimics (mimics NC as control) or miR-223 inhibi...

Stability of GC-Rich and GC-Poor mRNA in Mammalian Cells

Grzegorz Kudla (16996)
Leszek Lipinski (16997)
Fanny Caffin (16998)
Aleksandra Helwak (16999)
Maciej Zylicz (17000)

February 2013

<p>HeLa cells were transfected with the indicated plasmids and after 20 h they were treated with 10 ...

Identification of the optimal anti-myostatin miRNAs in 293FT cells.

Bushuai Zhong (632415)
Yanli Zhang (309699)
Yibo Yan (632416)
Ziyu Wang (451367)
Shijia Ying (632417)
Mingrui Huang (632418)
Feng Wang (44414)

September 2014

<p>Each of the four pD-miRNA plasmids was co-transfected with C1-MSTN into 293FT cells. Protein leve...

Higher expression of miR-351 modulates the expression of the nucleus intermediate filament lamin-B1.

Akira Sato (210501)
Takuya Omi (2608192)
Akihiro Yamamoto (2608189)
Akito Satake (2301061)
Akiko Hiramoto (2016034)
Mitsuko Masutani (78971)
Sei-ichi Tanuma (77409)
Yusuke Wataya (452256)
Hye-Sook Kim (452255)

April 2016

<p>(A) At 48 hours after transfection with the vehicle, non-silencing siRNA (NSsi) or the mature miR...

Effects of miR-137 on the IGFBP-5 expression in VSMCs.

Jin Pan (411619)
Kai Li (205203)
Wei Huang (36889)
Xiaoqing Zhang (80417)

October 2017

<p>(A) The relative mRNA expression levels of IGFBP-5 in VSMCs after miR-137 mimics, miR-137 inhibit...

miR-182 downregulates the expression of MITF and c-Met.

Dongsheng Yan (146499)
Xiang Da Dong (146502)
Xiaoyan Chen (146508)
Shasha Yao (146515)
Lihua Wang (14548)
Jiao Wang (47178)
Chao Wang (146527)
Dan-Ning Hu (146536)
Jia Qu (28271)
LiLi Tu (3560126)

July 2012

<p>(A) MITF expression in M23 and SP6.5 cells after transfection with miR-182 was dramatically reduc...

Western blot showing NF-κB p65, E2A, FOXO1, FOXP1, RAG expression in epithelial cancer cells.

Zhengshan Chen (126192)
Yanna Xiao (349235)
Junjun Zhang (2035)
Jing Li (10611)
Yuxuan Liu (349237)
Yingying Zhao (349236)
Changchun Ma (349239)
Jin Luo (349243)
Yamei Qiu (349241)
Guowei Huang (129576)
Christine Korteweg (126198)
Jiang Gu (126204)

February 2013

<p>Raji cell was used as a positive control. GAPDH was used as an internal control.</p

Reduction in MYCN protein levels in NB cells after treatment with small molecules.

Inga Müller (568256)
Karin Larsson (568257)
Anna Frenzel (194997)
Ganna Oliynyk (568258)
Hanna Zirath (195000)
Edward V. Prochownik (163628)
Nicholas J. Westwood (254891)
Marie Arsenian Henriksson (195006)

May 2014

<p>Western blot analysis of MYCN expression in SK-N-BE(2) cells treated for 48 hours with the indica...

HDAC4 and FGFBP1 expression levels in C2C12 cells transiently transfected with miRNA-206.

Valeria Valsecchi (494030)
Marina Boido (306249)
Elena De Amicis (494031)
Antonio Piras (347544)
Alessandro Vercelli (139469)

June 2015

<p>Panels A-B, representative Western blots displaying HDAC4 protein levels in C2C12 cells transfect...

miR-103 expression analysis in IGF-1 stimulated mouse small intestinal crypt cells.

Yalin Liao (361471)
Bo Lönnerdal (361472)

February 2013

<p>(A) microrarray analysis of total miRNA expression in crypt cells after stimulation with IGF-1 fo...