RNA interference-mediated gene silencing in murine T cells: and validation of proinflammatory target genes-5

stealth™ siRNAs (S#1, S#2, and S#3) by using nucleofection. Cell lysates were prepared 24 and 48 h after transfection and ZAP70 expression was analyzed by RT-PCR (A) and Western blot analysis (B). As control cells were mock transfected ("-", no siRNA added), or transfected with a control siRNA pool (Ctrl). (A) RT-PCR of ZAP70 mRNA expression in transfected T cells determined 24 h after nucleofection as fold expression of the house keeping gene β-actin. (B) Western blot analysis of transfected T cells 48 h after nucleofection with siRNAs indicated in (A). Protein knockdown of ZAP70 was detected by ZAP70-specific antibody. β-actin was used as loading control. Shown are the mean values of triplicates of a representative experiments of two independent experiments.<p><b>Copyright information:</b></p><p>Taken from "RNA interference-mediated gene silencing in murine T cells: and validation of proinflammatory target genes"</p><p>http://www.biosignaling.com/content/6/1/3</p><p>Cell communication and signaling : CCS 2008;6():3-3.</p><p>Published online 6 Aug 2008</p><p>PMCID:PMC2517589.</p><p>