Effect of siRNA-mediated silencing of ZFP36 on T cell activation, proliferation, and survival.

<p>PBMCs from healthy donors were transfected by nucleofection with non-specific siRNA (NC siRNA) or ZFP36-specific siRNA (ZFP36 siRNA). <b>A.</b> Expression of CD25 and CD69 in CD4⁺ and CD8⁺ T cells was measured 24 h after stimulation with anti-CD3/CD28. Dotted lines: un-stimulated cells; solid lines: stimulated cells; grey lines: NC siRNA-transfected cells; black lines: ZFP36 siRNA-transfected cells. <b>B.</b> CFSE dilution of labeled CD4⁺ and CD8⁺ T lymphocytes. Dotted lines: cells transfected with NC siRNA; solid lines: cells transfected with ZFP36 siRNA. <b>C.</b> Cell cycle status of ZFP36 siRNA-transfected T cells. Cells were stained with PI. Solid lines: cells transfected with NC siRNA; dotted lines: cells transfected with ZFP36 siRNA. <b>D.</b> Apoptotic rates of ZFP36 siRNA-transfected T cells as determined by Annexin V staining. Percentages represent the frequency of Annexin V⁺ cells within the CD4⁺ and CD8⁺ T cell populations of PBMCs transfected with NC siRNA or ZFP36 siRNA.