Representative immunofluorescence microphotographs of NSCLC samples (Fig. 1A: ADC and Fig. 1B:SCC) with high SOX2 levels and negative SOX2.

<p>The upper panels show SOX2 fluorescence (red channel) with characteristic nuclear staining pattern (left) and lack of SOX2 positivity (right). The lower panel shows cytokeratin immunoreactivity in the tumor compartment (AE1/AE3, green channel) and nuclear staining with DAPI (blue channel).