SOX2 is involved in the WNT signaling pathway in colorectal cancer cells.

<p>(A) Immunofluorescence images of SW620-mock and SW620-shRNA-SOX2 cells stained with anti-beta-catenin antibody. Secondary antibody was conjugated with CY3. Cell nuclei were stained with Hoechest 33258. The top 6 panels are in 100× magnification and the lower 6 panels are in 400× magnification. (B) Western blot analysis of beta-catenin expression using cytoplasmic and nuclear fractions of SW620-mock and SW620-shRNA-SOX2 cells. Alpha-tubulin and Lamin B were used as loading controls for the cytoplasmic and nuclear fractions respectively. The right panel showed the band intensities (left panel) quantified by the Quantity One software (Bio-Rad). (C) LEF1/TCF1-luciferase activity in SW620-mock and SW620-shRNA-SOX2 cells. (D) Quantitative real time PCR (upper panel) and western blot (lower panel) analyses of LEF1 in SW620-mock and SW620-shRNA-SOX2 cells. GAPDH was used as a loading control. (E) Quantitative real time PCR analysis of c-Myc gene in SW620-mock and SW620-shRNA-SOX2 cells. (F) Quantitative real time PCR analysis of cyclin D1 expression in SW620-mock and SW620-shRNA-SOX2 cells.