Anti-MOG antibodies generated in WT and AID^−/− mice.

<p>WT and AID^−/− mice were immunized with recombinant human MOG (rhMOG) and titres of anti-MOG Ab were evaluated at day 15 (peak of disease). As expected, AID^−/− mice only made anti-MOG Ab of the IgM isotype, whereas WT mice produced anti-MOG IgG1 and IgG2c Ab (and very little IgM at this time point). The average OD for each mouse at a set concentration of serum (half-way point of the curve) is depicted, and raw OD values can be seen in Supplemental <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061478#pone.0061478.s003" target="_blank">Figure S3</a>. In this experiment, 5–6 mice per group were tested and an additional experiment tested 8–12 mice.