γHV-68 EAE mice show increased levels of pro-inflammatory cytokines and chemokines and a decreased anti-MOG IgG1/IgG2a ratio in the serum.

<p>Mice were infected i.p. with γHV-68 (black bars) or MEM only (open bars). Five weeks p.i., EAE was induced. At day 10, 15 and 28 post EAE induction blood was harvested through a cardiac puncture and the levels of different (<b>A</b>) cytokines (day 28 shown here) and (<b>B</b>) chemokines (day 10 shown here) were evaluated using BD Cytometric Bead Array kits. Data from day 28 are shown for both IFN-γ and TNF-α; similar results were obtained at day 10 and 15 post EAE induction. Three-two separate experiments for each time point with 3–6 mice/group. Data were analyzed with t-test: *** p<0.001; ** p<0.01, * p<0.05. (<b>C–D</b>) Serum was harvested at day 28 post EAE and the levels of (<b>C</b>) total anti-MOG IgG (left panel), total anti-brain extract IgG (right panel) and (<b>D</b>) anti-MOG IgG1 and IgG2a were quantified through ELISA. Two separate experiments with 8 mice/group. Data were analyzed with t-test: ** p<0.01; and Mann-Whitney U test: * p<0.05.