Cytokine profile of immune splenocytes from each experimental group infigure 1 (20 independent splenocyte populations) activated with UV-inactivated <i>C. muridarum.</i>

<p>3×10⁶ splenocytes were activated with 7.5×10⁶ UV-inactivated <i>C. muridarum</i> (multiplicity of 2.5 IFU per splenocyte) or mock-activated with equivalent volume of SPG buffer (con = control) in triplicate wells. Supernatants were collected at 72 h and analyzed by ELISA. Data representing each mouse strain/treatment condition were aggregated and compared to the aggregated data for the untreated C57BL/6J mice. Comparisons were made for each cytokine. There were no statistically significant differences in cytokine polarization between wild type and perforin knockout mice with or without MLA treatment.