Splenic cellular antigen-specific IFN-γ response following immunization.

<p>C57BL/6 mice (3 mice/group) were treated i.n. with <i>C. pneumoniae</i> (1×10⁶ IFU/mouse), or <i>C. muridarum</i> (0.5×10³ IFU/mouse) in 25 µl of PBS, or PBS alone (mock). On day 14, animals were euthanized and splenocytes were tested by ELISA for antigen-specific IFN-γ production against <i>C. mur</i>, <i>C. tra,</i> or the unrelated antigen BSA. Cells cultured in media alone were used to evaluate baseline cytokine production from isolated splenocytes. Results are expressed as mean ± SEM of IFN-γ production per culture condition. *Significant (P<0.05, ANOVA) differences in IFN-γ production from splenocytes of <i>C. pne</i> versus <i>C. mur</i> immunized mice upon stimulation with <i>C. mur</i> or <i>C. tra</i>. Results are representative of two independent experiments.