The levels of IFN-γ, IL-2, and TNF-α secreted by splenocytes of differently immunized mice.

<p>Three weeks after the last immunization, splenocytes were obtained from each mouse of the differently vaccinated groups (n = 3). 2.5 × 10⁶ cells were added into each well of 24-well microtiter plates and incubated with A1D4 protein (10 μg/mL), PPD (10 μg/mL, positive control), or complete RPMI1640 medium (negative control) for 24 h (for IL-2 detection) or 72 h (for IFN-γ and TNF-α detection) at 37°C, respectively. The cytokine concentrations in the suspension were detected with commercial ELISA kits. The results are shown as mean ± SD (pg/mL) and the bar indicates <i>p</i><0.05. Similar results were obtained from two independent experiments.