IFN-γ production in mice immunized with killed vaccine and baculoviruses.

<p>Two weeks after the final immunization, splenocytes from mice injected intramuscularly with PBS, killed vaccine, AcHERV-HA, or AcHERV-VLP were collected and evaluated for the number of IFN-γ secreted spots from pH1N1-specific T cells in splenocytes. ELISPOT assays were performed using two randomly selected samples from each group (2/12). Values in parentheses indicate number of mice tested/total number of mice immunized in each group. All experiments were run in triplicate. The data shown are actual number of IFN-γ spot as a scatter dot and mean value as a line. Statistical analysis showed that data were significant with <i>p</i><0.05 (ANOVA and two-tailed Student’s <i>t</i>-test): killed vaccine and AcHERV HA groups were compared with AcHERV VLP group.