Measurement of cytokines expression in immunized mice splenocytes.

<p>Graphs showing concentrations of IL-2 <b>(A);</b> TNF-α <b>(B);</b> IFN-γ <b>(C)</b>; IL-4 <b>(D)</b> and IL-10 <b>(E)</b> in picograms per millilitre. Each bar represents the average of 10 mice/group ± S.D. and is representative of three independent experiments. Analysis was done by one way ANOVA, (Fisher LSD Method). ****P < 0.001 (significance between 72 and 48 hrs); ^<b>#</b>P < 0.001(significance with respect to control); ^<b>¤</b>P < 0.001(significance with respect to 10 μg inactivated CHIKV); *P < 0.05, **P < 0.01, ***P < 0.001(significance with respect to 20 μg inactivated CHIKV).